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What property of a compound light microscope enables a person to change objectives without major focusing adjustments?Resolving powerMagnificationParfocalFocusingResolution

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How does closing the iris diaphragm affect image brightness and contrast?Decreases bothIncreases bothDecreases brightness and increases contrastIncreases brightness and decreases contrastDecreases brightness but has no effect on contrast

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Which of the following objectives has the shortest working distance?4X10X45X100X

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What effect will changing from the low power objective to the high power objective have on the field of view of a specimen?IncreaseDecreaseNo effect

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If the letter p is placed under the microscope in the normal reading position, which of the following orientations of the letter would the viewer see?bpqd

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Which of the following procedures should be done just prior to adjusting the diopter, when using the compound microscope?Clean all lenses with lens paperTurn the light onAdjust the iris diaphragmAdjust the fine focus

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Two parts of the microscope that regulate the amount of light visible through the ocular are thefine and coarse adjustment knobs.ocular and objective lenses.illuminator and revolving nosepiece.condenser and iris diaphragm.

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Initial focusing of any slide is done under thescanning or low power objective.high power objective.oil immersion objective.depends upon the size of the specimen.

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Under which magnification would the distance between the objective and the specimen being observed be the least?40X100X450X1000X

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What is the total magnification produced by a microscope, using a 10X ocular lens and a 10X objective lens?10X20X100X1000X

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Which of the following describes proper microscope care and technique?Be sure to carry the microscope upright, with one hand on the arm and the other under the base.To protect the optics of the microscope, place it down gently and don’t drag it across the tabletop.Always begin focusing with the lowest power lens available.Only use the coarse focus knob with the scanning and low power lenses.All the above are examples of correct microscope care and technique.

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Turning which of the following knobs moves the objective lenses the greatest vertical distance?Coarse focus knobFine focus knob

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Resolution is the ability to see two objects as separate.TrueFalse

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Which of the following regulates the amount of light passing through the slide specimen on the microscope stage?NosepieceObjective lensIris diaphragm leverFine focus knob

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An increase in magnification causesa decrease in the field of view.a decrease in the working distance.a decrease in the depth of field.all of the above.

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Working distance is thedistance from your house to your job.distance the microscope nosepiece travels using the coarse focus knob.distance from the bottom of the objective lens to the specimen.distance the specimen can travel across the microscope stage.

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When adjusting a binocular microscope for differences between right and left eyes, focus the microscope with the fine adjustment while viewing only with the right eye (fixed ocular).TrueFalse

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When using the oil immersion lens, you should raise the objective lenses for easy application of an oil droplet to the slide.TrueFalse

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Why must a small object seen through the ocular lens be centered in the field of view before changing to a higher power objective lens?To prevent damage to the objective lensTo compensate for the decrease in resolving power of the lensTo prevent its disappearance due to the decrease in the field of viewTo prevent eyestrain

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A parfocal microscope allows you to quickly focus using the low power or scanning lens and then switch to a higher power lens with only a minimal adjustment of the fine focus knob.TrueFalse

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Why is it necessary to adjust the amount of light after changing objective lenses?Higher magnification lenses require more light.Proper lighting is needed to see specimen details.The lens aperture decreases with higher magnification lenses.All of the above.

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Which objective would you use to locate a specimen on a slide?Lowest power availableHigh powerOil immersionAny of the objectives could be used with equal success

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Which of the following parts of the microscope would increase the depth of field?OcularHigh power objectiveFine focusLow power objective

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24 of 25

Depth of field is best demonstrated witha slide showing the letter e.a slide containing overlapping threads.the light turned completely off.the oil immersion lens.

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25 of 25

When the letter e slide is viewed with the microscope, it appearsright side up.right side up and backwards.upside down.upside down and backwards.

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